IBD bioanalysis is encompassed of many different matrices including serum, colon tissues and feces. Fecal bioanalysis can be particularly useful since it provides a direct and non-invasive window into gut biology. Multiple biomarkers that can be detected in fecal samples can provide crucial information of gut biology both with and without drug on-board. Currently fecal bioanalysis can suffer from many gaps including matrix effects due to unknows and variable composition of fecal which can lead to reduced sensitivity, precision and high assay CV leading to limited reproducibility of the assays.
We believe this presentation will benefit gut biologists and pharmacists to get insight into how to mitigate matrix effects in feces while studying biomarkers and therapeutic modalities for IBD. Additionally bioanalysts working in other therapeutic areas will also benefit from this platform comparison to enhance sensitivity and reduce matrix interference in complex matrices.
Fecal sample bio-analysis can be crucial in IBD to understand bio-distribution of therapeutic molecules as well as study various disease biomarkers. The inherent complexity of fecal matrices and pronounced matrix interferences present substantial challenges for the development of precise and sensitive bio-analytical methods. Our study systematically evaluated novel and different bio-analytical platforms and mitigation strategies to reduce matrix interference in fecal samples, thereby enhancing assay sensitivity. Specifically, we compared the gold standard ligand binding assays with ultra-sensitive immunoassay and electrophoresis techniques. Additionally, we compared fecal sample homogenization methods and their impact on analyte detection using bioanalytical strategies.Our findings and conclusions are useful for developing both PK and PD assessment for different modalities that are delivered within gut.
Learning Objectives:
Upon completion, participant will be able to understand complexities in fecal sample bioanalysis and learn bioanalytical tools at hand to mitigate matrix interference challenges to improve sensitivity in complex matrices
Upon completion, participant will be able to understand how tissue homogenization process and various parameters like storage condition, temperature and speed can impact bioanalytical assessments
Upon completion, participant will be able to understand different strategies that can be applied to less studied complex matrices to improve precision, specificity and sensitivty
