Description: A trastuzumab-siRNA conjugate is used as a detailed case study, demonstrating native MS analysis for oligonucleotide-antibody ratio determination, detection of building blocks (sense/antisense oligonucleotides), peptide mapping with ~100% sequence coverage, conjugation site localization via EAD vs. CID, labile PTM mapping, and amino acid isomer differentiation.
Learning Objectives:
Have a better understanding of AOC characterization.
Recognize advantages of complimentary analytical technologies
Identify the right tools for isomer characterization
